Because it reveals new information about alternative treatment approaches of diseases ( Kashyap et al. Therefore, it is important to clarify the action mechanisms of natural medicines. Expensive synthetic drugs have led developing and poor countries to cheaper drugs. Various diseases are now being treated with cost-effective herbal remedies. This study showed that fisetin has the liver protective effects by increasing GSH, decreasing inflammatory mediators and CYP2E1.įisetin rat paracetamol hepatotoxicity TNF-α CYP2E1 Histopathological examinations showed that fisetin has hepatoprotective effects. The TNF-α, NFκB and CYP2E1 gene expressions were significantly lower in both doses of the fisetin groups compared with the PARA group. In addition, SOD activity and GSH levels increased, and the MDA level decreased with the treatment of fisetin. Fisetin administration significantly decreased the ALT, AST and ALP levels in a dose dependent manner. Histopathological examinations were also performed. Serum ALT, AST and ALP levels were measured. Tumor necrosis factor-alpha (TNF-α), NFκB and CYP2E1 mRNA levels and Superoxide dismutase (SOD) activity, glutathione (GSH) and malondialdehyde (MDA) levels of livers were determined. The rats were sacrificed 24h after the Paracetamol administration. Paracetamol was administered orally at a dose of 2 g/kg for induce hepatotoxicity 1 h after the fisetin and NAC treatments. Fisetin was administered at 25 and 50 mg/kg doses. We aimed to evaluate the possible protective effect of fisetin on paracetamol-induced hepatotoxicity. Fisetin has wide pharmacological effects such as anticancer, antiinflammatory and antioxidant. Paracetamol is one of the drugs that cause hepatic damage.

N acetylcysteine is antidote for